Artificial repressors for controlling gene expression in bacteria
نویسندگان
چکیده
منابع مشابه
Artificial repressors for controlling gene expression in bacteria.
Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.
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The progress of synthetic biology allows one to design artificial repressors that inhibit selected genes. Combination of repressors enables construction of NOR logical gates that could form the foundation for information processing within cells. The theoretical potentials and limitations of constructing NOR gates were analyzed. They could be experimentally realized in bacterial cells. The numbe...
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SUMMARY DNA-binding repressor proteins that govern transcription initiation in response to end products generally regulate bacterial biosynthetic genes, but this is rarely true for the pyrimidine biosynthetic (pyr) genes. Instead, bacterial pyr gene regulation generally involves mechanisms that rely only on regulatory sequences embedded in the leader region of the operon, which cause premature ...
متن کاملTranscriptional repressors: multifaceted regulators of gene expression.
Through decades of research it has been established that some chromatin-modifying proteins can repress transcription, and thus are generally termed 'repressors'. Although classic repressors undoubtedly silence transcription, genome-wide studies have shown that many repressors are associated with actively transcribed loci and that this is a widespread phenomenon. Here, we review the evidence for...
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The repertoire of cis-regulatory elements has increased to a level of sophistication that offers considerable spatial and temporal control over transgene expression. Recent advances made with transgenes have revealed that the control of their expression is also influenced by factors that range from transgene copy number and arrangement to nuclear architecture and chromosomal location. These fac...
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ژورنال
عنوان ژورنال: Chem. Commun.
سال: 2013
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c2cc37107c